Chickens were given various fowlpox vaccines on food pellets–a commercial vaccine (strain M), and the same strain after a single passage on chorio-allantoic membrane or in chicken embryo fibroblasts. All three oral vaccines induced antibodies at levels similar to those induced by commercial strain M administered to the wingweb.
The oral vaccine derived from chorio-allantoic membrane gave protection similar to that obtained with vaccine administered by the wingweb, but this required a thousandfold more virus.
The oral vaccination against Fowlpox was investigated via drinking water containing the F132-c strain of Fowlpox virus to be effective even though the vaccine virus-titer was 10(4) TCID (50)/dose each time. When the virus-titer of the F132-c strain was 10(4-5 )TCID(50)/dose per single drinking water vaccination, 90% or more of chickens were not protected, however, they were protected when vaccinated twice via drinking water. A weak immune response occurred by a slight infection after the first vaccination, and due to memory cells, a booster could work well after the second vaccination. These results suggest the possibility of reducing both the amount of virus required for a vaccine via drinking water and the labor cost in the field.
The vaccine is recommended for vaccination of healthy chickens aged 8 weeks or older but at least 4 weeks prior to start of lay. When used as indicated, it will aid in preventing the clinical signs caused by the virulent field strains of fowl pox virus.
Tear off the aluminum seal from the vial containing the freeze dried virus. Lift off the rubber stopper. Remove the seal and stopper from the diluent bottle. Each diluent bottle contains 10 ml of diluent. Pour half of the diluent into the vial containing the freeze dried virus. Replace the rubber stopper and shake the vaccine vial. Pour the partly dissolved vaccine into the diluent bottle to mix with rest of the diluent. Replace the rubber stopper and shake vigorously until the vaccine is dissolved completely. The vaccine is now ready for administration by the wing-web method. For administering the vaccine, hold the bird and spread the underside of one wing outward. Insert the double needle applicator into the vaccine bottle, wetting or charging both needles. Pierce the web of the exposed wing with the double needle applicator charged with vaccine. Insert the double needle applicator into the vaccine vial again and proceed to vaccinate the next bird. During vaccination avoid hitting large blood vessels, bones and the wing muscles with the double needle applicator. Do not inject in any other site except the exposed wing web.
Fowl Pox Vaccine Caution
Do not spill or splatter the vaccine. Burn containers, unused vaccine and accessories prior to disposal. Do not over dilute the vaccine or otherwise extend the dosage. Store unopened vaccine vials at not over 45° F or 7° C. Do not vaccinate within 21 days of slaughter or 4 weeks prior to start of lay. The vaccinated chickens should not be placed on contaminated premises. All susceptible chickens on the same premises should be vaccinated at the same time. If this is not possible, then strict isolation and separate caretakers should be employed for non-vaccinated chickens. Efforts should be made to reduce stress conditions at the time of vaccine administration.
Price of Oral Fowl Pox Vaccine