Vaccine For Marek’s Disease In Chickens

Marek’s disease is highly contagious and readily transmitted among chickens. The virus matures into a fully infective, enveloped form in the epithelium of the feather follicle, from which it is released into the environment. It may survive for months in poultry house litter or dust. Dust or dander from infected chickens is particularly effective in transmission. Once the virus is introduced into a chicken flock, regardless of vaccination status, infection spreads quickly from bird to bird.

Infected chickens continue to be carriers for long periods and act as sources of infectious virus. Shedding of infectious virus can be reduced, but not prevented, by prior vaccination. Unlike virulent strains of Marek’s disease virus, which are highly contagious, turkey herpesvirus is not readily transmissible among chickens (although it is easily transmitted among turkeys, its natural host). Attenuated Marek’s disease virus strains vary greatly in their transmissibility among chickens; the most highly attenuated are not transmitted. Marek’s disease virus is not vertically transmitted.


This vaccine is recommended for in ovo vaccination of 18- to 19-day-old embryonated chicken eggs. For in ovo vaccination, only healthy embryonated eggs should be used. This vaccine is also recommended for subcutaneous vaccination of healthy one-day-old chickens. It is essential that the chickens be maintained under good environmental conditions and that exposure to disease viruses be reduced as much as possible.

Preparation of Marek’s Vaccine

1. Dilute the vaccine only as directed, observing all precautions and warnings for handling.

2. Remove from the liquid nitrogen only the ampules that are going to be used immediately. Move quickly, but carefully.

3. Place the ampule(s) in a large, clean container of water at 68° to 86°F (20° to 30°C) to thaw ampule quickly. Thaw the entire contents. Gently swirl the ampule to disperse contents. Break ampule at its neck and quickly proceed as described below.

4. Use 200 ml of sterile diluent for each 1,000 doses of vaccine to be injected subcutaneously or for each 4,000 doses of vaccine to be administered in ovo. Remove the cover from the diluent container. Draw contents of the ampule into a sterile 10 ml syringe fitted with an 18 to 20 gauge needle. Slowly add the contents of the vaccine ampule to the appropriate volume of diluent. Withdraw a small amount of the diluent, rinse the ampule once and add this to the vaccine-diluent mixture. Mix the contents of the diluent container thoroughly by swirling and inverting the container. Do not shake vigorously.

5. Keep the container of diluted vaccine in an ice bath. Use the vaccine-diluent mixture immediately. Agitate as needed to ensure a uniform suspension of the cells.

Use Of Vaccine For in Ovo Administration

Before initiating in ovo vaccination, carefully read and follow the egg injection system operator’s manual. Failure to follow instructions may result in personal injury, embryonic morbidity and mortality.

1. Sanitize the egg injection system before and after use.

2. Inject a 0.05 ml dose into each embryonated egg.

3. Use the entire contents of the vaccine container within one hour after mixing the vaccine with the diluent.

Use Of Vaccine For Subcutaneous Injection

IMPORTANT: Sterilize vaccinating equipment by autoclaving a minimum of 15 minutes at 250°F (121°C) or boiling in water for at least 20 minutes. Never allow chemical disinfectants to come in contact with vaccinating equipment.

1. Use a sterile automatic syringe with a 20 to 22 gauge 3/8” to 1/2” needle that is set to accurately deliver 0.2 ml per dose. Check the accuracy of delivery several times during the vaccination procedure.

2. Inject chickens under the loose skin at the back of the neck (subcutaneously), holding the chicken by the back of the neck just below the head. The loose skin in this area is raised by gently pinching with the thumb and forefinger. Insert the needle beneath the skin in a direction away from the head. Inject 0.2 ml per chicken. Avoid hitting the muscles and bones in the neck.

3. Use the entire contents of the vaccine container within one hour after mixing the vaccine with the diluent.

Prices of Vaccine For Marek’s Disease In Chickens


Leave a Comment

This site uses Akismet to reduce spam. Learn how your comment data is processed.